Inhibition of epigallocatechin-3-gallate on scald-induced macrophage inflammatory protein-2 expression in the skin of mice

doi:10.3969/j.issn.0529-1356.2013.03.007

Abstract

Abstract:

Objective To investigate influence of epigallocatechin-3-gallate (EGCG) on expression of macrophage inflammatory
protein-2(MIP-2) induced by locally scalded skin in mice. Methods Fity-two Kunming mice were divided into the control, scalded
skin, and EGCG groups. Forty-eight mice were used to establish a skin scalded model. The real time quantitative PCR (qRT-PCR)
and ELISA were used to evaluate MIP-2 expression in cutaneous tissue at both the mRNA and protein expression levels. Results
Both MIP-2 mRNA and protein levels in cutaneous tissue at injury sites increased after skin was scalded, but both decreased after
the scalded skin was treated by 0.2g/kg EGCG. Conclusion The data suggest that 0.2g/kg EGCG may suppress MIP-2 expression stimulated
by local skin scald, and decrease inflammatory reaction induced by MIP-2 release in cutaneous tissue at injury sites, thus EGCG
is able to promote the repair of skin injury elicited by scald.

Key words: Scald, Skin, Epigallocatechin-3-gallate, Macrophage inflammatory protein-2, qRT-PCR, ELISA, Mouse

CLC Number:

LI Jia-wen CUI Hai-yan ZHANG Dong-fang XIAO Peng LI Wei-guo*. Inhibition of epigallocatechin-3-gallate on scald-induced macrophage inflammatory protein-2 expression in the skin of mice[J]. AAS, 2013, 44(3 ): 330-333.

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